Protein recovery from blood plasma by precipitation with polyuronates
โ Scribed by A. P. IMESON; P. R. WATSON; J. R. MITCHELL; D. A. LEDWARD
- Book ID
- 108803053
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 494 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0950-5423
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๐ SIMILAR VOLUMES
Precipitation with PEG and partitioning in PEG/dextran aqueous twophase systems are applied to the purification of protein C, a human plasma protein with a key role in anti-coagulation. Tandem application of precipitation followed by partitioning yields a purification factor for protein C greater th
A new, improved method is described, which takes advantage of the low solubility of potassium dodecyl sulfate (KDS), to concentrate sodium dodecyl sulfate-solubilized proteins from nanomolar solutions by KDS precipitation.The method was applied to proteins differing in M, and PI. The effect of varyi