Effective recovery by KCl precipitation of highly diluted muscle proteins solubilized with sodium dodecyl sulfate
✍ Scribed by Prof. Ugo Carraro; Corrado Rizzi; Marco Sandri
- Book ID
- 102835093
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 810 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
A new, improved method is described, which takes advantage of the low solubility of potassium dodecyl sulfate (KDS), to concentrate sodium dodecyl sulfate-solubilized proteins from nanomolar solutions by KDS precipitation.The method was applied to proteins differing in M, and PI. The effect of varying KC1, and the pH and/or concentration of the buffer on KDS-protein precipitation was studied. These parameters may be chosen to allow selective repartition of specific proteins in the pellet or supernatant. After precipitation, the hundred-or thousand-fold concentrated solutes are easily resuspended in small volumes of any required medium. Though initially experimented to recover muscle proteins from effluents of electroendosmotic preparative gel electrophoresis, the method proved to be of general interest as a powerful tool to recover proteins from highly diluted solutions.