Purification of protein C from human plasma by precipitation and aqueous two-phase partitioning

✍ Scribed by Casimir N. Ryzewski; James T. Hsu

• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 479 KB
• Volume: 7
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00153751

✦ Synopsis

Precipitation with PEG and partitioning in PEG/dextran aqueous twophase systems are applied to the purification of protein C, a human plasma protein with a key role in anti-coagulation. Tandem application of precipitation followed by partitioning yields a purification factor for protein C greater than that using either process individually. The results are discussed using the hypothesis that the mechanism of solvation of protein C by PEG is similar, while that for total plasma proteins is different, in the two processes.