𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Separation and purification of recombinant proteins from Escherichia coli with aqueous two-phase systems

✍ Scribed by J.A. Asenjo; R.E. Turner; S.L. Mistry; A. Kaul

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
752 KB
Volume
668
Category
Article
ISSN
1873-3778

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Aqueous two-phase systems for the recove
Aqueous two-phase systems for the recovery of a recombinant viral coat protein from Escherichia coli
✍ Marco Rito-Palomares; Anton Pβ€ŠJ Middelberg πŸ“‚ Article πŸ“… 2002 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 105 KB πŸ‘ 1 views

## Abstract In this study the use of an aqueous two‐phase system (ATPS) following the direct chemical extraction of a recombinant viral coat protein, from the cytoplasm of __Escherichia coli__, is evaluated. The driving force is the need to establish an economically‐viable process for the manufactu

Purification of recombinant proteins usi
Purification of recombinant proteins using thermoseparating aqueous two-phase system and polymer recycling
✍ Josefine Persson; Lena NystrΓΆm; Hans Ageland; Folke Tjerneld πŸ“‚ Article πŸ“… 1999 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 169 KB πŸ‘ 2 views

Aqueous two-phase systems containing two polymers are a mild separation method for biomolecules due to the high concentration of water (75-90% ) in the phases. The phase systems have been further developed by introduction of thermoseparating polymers which make recycling of phase components possible

Purification of recombinant and human ap
Purification of recombinant and human apolipoprotein A-1 using surfactant micelles in aqueous two-phase systems: Recycling of thermoseparating polymer and surfactant with temperature-induced phase separation
✍ Josefine Persson; Lena NystrΓΆm; Hans Ageland; Folke Tjerneld πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 299 KB πŸ‘ 2 views

An effective system has been developed for purification of apolipoprotein A-1 from Escherichia coli fermentation solution and human plasma using aqueous two-phase extraction and thermal-phase separation. The system included non-ionic surfactants (Triton or Tween) and as top phase-forming polymer a r