Two-polymer aqueous phase systems are described in which ribosomes selectively partition into one of the phases. One of the phase systems is used to determine rapidly and conveniently binding of thiostrepton and erythromycin to Escherichia co/i ribosomes under equilibrium conditions.
Purification of proteins by aqueous two-phase partition in novel acrylic co-polymer systems
β Scribed by Peter Hughes; Christopher R. Lowe
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 696 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0141-0229
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π SIMILAR VOLUMES
In this study we show that proteins can be partitioned and separated in a novel aqueous two-phase system composed of only one polymer in water solution. This system represents an attractive alternative to traditional two-phase systems which uses either two polymers (e.g., PEG/dextran) or one polymer
Aqueous two-phase systems containing two polymers are a mild separation method for biomolecules due to the high concentration of water (75-90% ) in the phases. The phase systems have been further developed by introduction of thermoseparating polymers which make recycling of phase components possible