Properties of riboflavin phosphates
β Scribed by Paolo Cerletti
- Publisher
- Elsevier Science
- Year
- 1959
- Tongue
- English
- Weight
- 391 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Phosphoric acid esters of riboflavin can be easily separated by reverse-phase high-performance liquid chromatography using eluants of 0.1 M ammonium formate in aqueous methanol. Commercial FMN preparations contained seven different flavin phosphates; the content of riboflavin S-phosphate was 70-7590
Alkaline phosphatase hydrolyzes riboflavin 4'-phosphate to produce riboflavin. This is converted to riboflavin 5'-phosphate, using riboflavin kinase, which reconstitutes apoglycolate oxidase to give hologlycolate oxidase. This enzyme catalyzes the oxidation of glycolate with simultaneous production
An asasy for alkaline phosphatase is described which is based on the hydrolysis of riboflavin phosphates (5'FMN or 4 F M N ) t o produce riboflavin. This is converted t o 5'FMN using riboflavin kinase. and then asayed using the bacterial bioluminescent system from Vibrio harveyi or V. fischeri. The