✦ LIBER ✦
Amplified colorimetric assay of alkaline phosphatase using riboflavin 4′-phosphate: A simple method for measuring riboflavin and riboflavin 5′-phosphate
✍ Scribed by Stuart Harbron; Henny J. Eggelte; Brian R. Rabin
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 504 KB
- Volume
- 198
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Alkaline phosphatase hydrolyzes riboflavin 4'-phosphate to produce riboflavin. This is converted to riboflavin 5'-phosphate, using riboflavin kinase, which reconstitutes apoglycolate oxidase to give hologlycolate oxidase. This enzyme catalyzes the oxidation of glycolate with simultaneous production of hydrogen peroxide which is detected via the formation of a colored product through the action of peroxidase. The system allows the detection of 4 amol after a 2-h incubation.