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Production of α-Amylase in Fed-Batch Cultures of vgb+ and vgb− Recombinant Escherichiacoli: Some Observations

✍ Scribed by Nader Enayati; Canan Tari; Satish J. Parulekar; Benjamin C. Stark; Dale A. Webster

Book ID
109388259
Publisher
American Institute of Chemical Engineers
Year
1999
Tongue
English
Weight
83 KB
Volume
15
Category
Article
ISSN
8756-7938

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✍ Canan Tari; Satish J. Parulekar; Benjamin C. Stark; Dale A. Webster 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 86 KB

Synthesis and excretion of ␣-amylase is investigated in batch cultures of Escherichia coli JM103[pMK57] (vgb -) and E. coli JM103[pMK79] (vgb + ). While total production and excretion of ␣-amylase were promoted in Luria broth (LB) (excretion being as high as 87%), cell-massspecific production of the