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Structured modeling of recombinant protein production in batch and fed-batch culture of baculovirus-infected insect cells

✍ Scribed by J. D. Jang; C. S. Sanderson; L. C. L. Chan; J. P. Barford; S. Reid


Book ID
110259688
Publisher
Springer
Year
2000
Tongue
English
Weight
222 KB
Volume
34
Category
Article
ISSN
0920-9069

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An amplified NS0 cell line transfected with a vector expressing a humanized monoclonal antibody (MAb) against CD-18 and glutamine synthetase (GS) was cultivated in a 1.5 L fed-batch culture using a serum-free, glutamine-free medium. Concentrated solutions of key nutrient components were fed periodic