Synthesis and excretion of α-amylase in vgb+ and vgb− recombinant escherichia coli: A comparative study

✍ Scribed by Canan Tari; Satish J. Parulekar; Benjamin C. Stark; Dale A. Webster

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 86 KB
• Volume: 59
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19980920)59:6<673::aid-bit3>3.0.co;2-c

✦ Synopsis

Synthesis and excretion of ␣-amylase is investigated in batch cultures of Escherichia coli JM103[pMK57] (vgb -) and E. coli JM103[pMK79] (vgb + ). While total production and excretion of ␣-amylase were promoted in Luria broth (LB) (excretion being as high as 87%), cell-massspecific production of the enzyme was promoted in M9 in bioreactor cultures and in LB in shake flask cultures. Low aeration and agitation rates and presence of starch were conducive to ␣-amylase synthesis in E. coli JM103[pMK79]. Two-stage bioreactor operating strategies that will improve ␣-amylase production are proposed. The potential of these strategies is demonstrated via two-stage shake flask cultures.