Production of pectinases byAspergillus nigerin solid state fermentation at high initial glucose concentrations
✍ Scribed by S. Solis-Pereyra; E. Favela-Torres; M. Gutiérrez-Rojas; S. Roussos; G. Saucedo-Castañeda; P. Gunasekaran; G. Viniegra-González
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 327 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1573-0972
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✦ Synopsis
Exopedinase
(exo-p) and endopectinase (endo-p) production by Aspergiflus niger CH4 in solid state culture was studied at initial glucose concentrations of 100, 250, 350 and 450 g/l. The highest activity of exo-p (35 U/g) was produced at 72 and 120 h in the medium containing 100 and 250 g glucose/l, respectively. The maximum endo-p activity (9 U/g) was produced at 72 h in the medium with 250 g glucose/l. The reduction in pectinase production at 350 and 450 g/l initial glucose concentration was due neither to repression of the synthesis of the enzyme nor to the glucose consumption rate of the strain but due to a drastic drop in pH of the medium.
📜 SIMILAR VOLUMES
Aspergillus niger cultures at high initial glucose concentration (up to 400 gO) on Amberlite as inert support were carried out. Citric acid was accumulated in the support showing high concentration (94.54 g/l) and productivity (1.35 g/1 h) without inhibition related to the presence of metals (Mn 2+,
Aspergillus niger CFTRI 30 produced 1.3 g citric acid/10 g dry coffee husk in 72 h solid-state fermentation when the substrate was moistened with 0.075 M NaOH solution. Production was increased by 17% by adding a mixture of iron, copper and zinc to the medium but enrichment of the moist solid medium