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Production of human-mouse chimeric antibody by high cell density perfusion culture

โœ Scribed by Yoshiharu Takazawa; Michiyuki Tokashiki

Publisher
Springer
Year
1989
Tongue
English
Weight
613 KB
Volume
2
Category
Article
ISSN
0920-9069

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โœฆ Synopsis

Two mouse myeloma cell lines which were transfected with chimeric mouse variable-human constant immunoglobulin heavy and light chain genes have been cultured at high cell density in a settling perfusion culture vessel to produce chimeric antibody specific for human common acute lymphocytic leukemia antigen (cALLA).

J558L transfectant proliferated well in a serum-free medium (ITES-eRDF) to a viable cell density of 3.7 x 107 cells/ml and produced chimeric antibody to a maximum value of 60 Ixg/ml in 120 ml scale vessel. X63Ag8.653 transfectant reached a density of 1.9 โ€ข 107 cells/ml in 1.2 1 scale vessel in serum supplemented medium (10% FCS-eRDF) and produced chimeric antibody which consisted of chimeric gamma and chimeric kappa chains to a maximum value of 5.8 gg/ml.

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