High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium
✍ Scribed by Michiyuki Tokashiki; Takami Arai
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 248 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0920-9069
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✦ Synopsis
Mouse-human hybridoma X87X ceils were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0 x 10 v cells/ml, when the specific perfusion rate was set to 2.3 vol day -1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.