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A high density culture system for the in-vitro production of human and mouse monoclonal antibodies

✍ Scribed by M. J. Munster; M. J. Kearns; U. Steegmans; U. Behrendt; M. J. Comer

Publisher
Springer
Year
1991
Tongue
English
Weight
260 KB
Volume
6
Category
Article
ISSN
1615-7605

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✦ Synopsis

A simple high density cell culture system is described which demonstrates many of the features of commercially available hollow-fibre systems, but without the need to invest in a dedicated system. The system has been shown to achieve product concentrations of up to 40-fold greater than that obtained in batch culture making gram production of MAbs possible with considerable saving on serum costs.

List of symbols

ELISA

FBS IgG IgM MAb pH pO2 RPMI1640 enzyme-linked immunosorbent assay foetal bovine serum immunoglobulin G immunoglobulin M monoclonal antibody negative common logarithm of the hydrogen ion concentration dissolved oxygen concentration Roswell Park Memorial Institute medium 1640

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