Yeast cells (Saccharomyces cerevX~iae) were immobilized in pectin gel, incubated 12 h at 30°C and then used for the continuous production of ethanol employing a wedge-shaped horizontal reactor and sugar cane molasses as the carbon source. Under steady state conditions the mean residence time was 1.6
Production of ethanol by yeasts immobilized in pectin
✍ Scribed by Antonio R. Navarro; María C. Rubio; Danley A. S. Callieri
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 312 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1432-0614
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✦ Synopsis
were immobilized by occlusion in pectin gel, no significant changes in their biological activity being observed. The ethanol production rate was found to be negatively affected by the diameter of the beads when the cell concentration in them was high. The fermentative activity was only slightly modified by pectin concentration. Cells maintained their growth capacity and the beads could be reutilized several times in succesive batch fermentations. After freeze-drying and storage at room temperature the beads retained both their morphological and mechanical characteristics as well as 93% of their initial fermentative activity.
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Immobilized yeast was encapsulated with cell-free calciumalginate gel by two-step preparation procedure. The volume of coated film decreased with increasing cell concentration. The encapsulation did not affect ethanol production and could prevent cell leakage from the gels.
## Abstract __Saccharomyces cerevisiae__ cells were immobilized in calcium alginate beads for use in the continuous production of ethanol. Yeasts were grown in medium supplemented with ethanol to selectively screen for a culture which showed the greatest tolerance to ethanol inhibition. Yeast beads