were immobilized by occlusion in pectin gel, no significant changes in their biological activity being observed. The ethanol production rate was found to be negatively affected by the diameter of the beads when the cell concentration in them was high. The fermentative activity was only slightly modi
Horizontal reactor for the continuous production of ethanol by yeasts immobilized in pectin
✍ Scribed by Antonio Navarro; Hugo Marangoni; Ignacio Magaña Plaza; Danley Callieri
- Publisher
- Springer Netherlands
- Year
- 1984
- Tongue
- English
- Weight
- 431 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
Yeast cells (Saccharomyces cerevX~iae) were immobilized in pectin gel, incubated 12 h at 30°C and then used for the continuous production of ethanol employing a wedge-shaped horizontal reactor and sugar cane molasses as the carbon source. Under steady state conditions the mean residence time was 1.6 h and the volumetric productivity 40 g EtOH/hl. The gas evolved was easily released. Successive batch incubation in a synthetic medium substantially restored the fermentative capacity of the beads already used in the continuous assay.
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In order to minimize the adverse effect of CO 2 gas in a packed bed immobilized yeast reactor, a fluidized bed reactor was used for the continuous production of ethanol from glucose. Immobilized yeast was prepared by entrapping whole cells of Saccharomyces cerevisiae within a Caalginate matrix. It
## Abstract __Saccharomyces cerevisiae__ cells were immobilized in calcium alginate beads for use in the continuous production of ethanol. Yeasts were grown in medium supplemented with ethanol to selectively screen for a culture which showed the greatest tolerance to ethanol inhibition. Yeast beads