Production of arachidonic acid byMortierellafungi
β Scribed by Kenichi Higashiyama; Shigeaki Fujikawa; Enoch Y. Park; Sakayu Shimizu
- Publisher
- The Korean Society for Biotechnology and Bioengineering
- Year
- 2002
- Tongue
- English
- Weight
- 266 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1226-8372
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A growth-inhibiting, aspirin-containing medium was developed to select arachidonic-acid-(ARA)-producing Mortierella species and to determine the fatty-acid content of 87 Mortierella strains. ARA was detected in 66 strains from 33 species and its production may prove useful for systematic studies on
Mortierella alpina S-17 cultivated in shaker flasks in the semisynthetic liquid medium with 3% glucose at 28 OC for 14 days accumulated 2.1 g/l of intracellular lipid rich in arachidonic acid (ARA). The content of AR4 was 50 % of the total fatty acids. The simple method for high pure methyl arachido
## Mortierelfa alpina grown in solid state fermentations on cereal substrates gave up to 16% lipid in the final biomass. Arachidonic was at 50% of total fatty acids, with a yield of 36 mg/g of original substrate. Microbial lipid production was successfully scaled up to use S-kg dry substrate batc