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Production of antithrombotic hirudin inGAL1-disruptedSaccharomyces cerevisiae

โœ Scribed by M.-D. Kim; T.-H. Lee; H.-K. Lim; J.-H. Seo

Publisher
Springer
Year
2004
Tongue
English
Weight
100 KB
Volume
65
Category
Article
ISSN
1432-0614

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The enriched mcditml based oll yeast nitrogen basc(YNB)incrcased hirudin svnthesis and secretion in recombinant SacchtTromyces cerevimae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342mg hirudind but supplementation of yeast extract increased the final hir