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Effects of medium composition on hirudin production in recombinantSaccharomyces cerevisiae

✍ Scribed by Chi-Min Choi; Myoung-Dong Kim; Sang-Ki Rhee; Jin-Ho Seo

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
223 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis

The enriched mcditml based oll yeast nitrogen basc(YNB)incrcased hirudin svnthesis and secretion in recombinant SacchtTromyces cerevimae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342mg hirudind but supplementation of yeast extract increased the final hirudin concentration to 461mg hirudin/l. The defined medium, however, produced the product protein with higher purity of 21% and hence will allow easy separation of secreted hirudin from other contaminated polypeptides present in the growth medium. In a continuous euluture, the defined medium yielded higher concentrations of cell mass and hirudin than the complex medium.

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