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Effect of galactose feeding on the improved production of hirudin in fed-batch cultures of recombinantSaccharomyces cerevisiae

โœ Scribed by K. Jagannadha Rao; Chul-Ho Kim; Bong Hyun Chung; Jung-Hoon Sohn; Sang-Ki Rhee

Publisher
Springer
Year
1998
Tongue
English
Weight
152 KB
Volume
19
Category
Article
ISSN
1615-7605

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The enriched mcditml based oll yeast nitrogen basc(YNB)incrcased hirudin svnthesis and secretion in recombinant SacchtTromyces cerevimae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342mg hirudind but supplementation of yeast extract increased the final hir