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Expression of hirudin in fed-batch cultures of recombinantSaccharomyces cerevisiae

✍ Scribed by Dong-Hoon Lee; Jin-Byung Park; Jin-Ho Seo; Eui-Sung Choi; Sang-Ki Lee

Publisher
Springer Netherlands
Year
1994
Tongue
English
Weight
227 KB
Volume
16
Category
Article
ISSN
0141-5492

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✍ Chi-Min Choi; Myoung-Dong Kim; Sang-Ki Rhee; Jin-Ho Seo πŸ“‚ Article πŸ“… 1996 πŸ› Springer Netherlands 🌐 English βš– 223 KB

The enriched mcditml based oll yeast nitrogen basc(YNB)incrcased hirudin svnthesis and secretion in recombinant SacchtTromyces cerevimae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342mg hirudind but supplementation of yeast extract increased the final hir