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Scale-up of recombinant hirudin production fromSaccharomyces cerevisiae

✍ Scribed by Chul Ho Kim; K. Jagannadha Rao; Duk Joong Youn; Sang Ki Rhee


Publisher
The Korean Society for Biotechnology and Bioengineering
Year
2003
Tongue
English
Weight
108 KB
Volume
8
Category
Article
ISSN
1226-8372

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Effects of medium composition on hirudin
✍ Chi-Min Choi; Myoung-Dong Kim; Sang-Ki Rhee; Jin-Ho Seo πŸ“‚ Article πŸ“… 1996 πŸ› Springer Netherlands 🌐 English βš– 223 KB

The enriched mcditml based oll yeast nitrogen basc(YNB)incrcased hirudin svnthesis and secretion in recombinant SacchtTromyces cerevimae in batch and fed-batch cultures. Fed-batch fermentation with the defined medium yielded 342mg hirudind but supplementation of yeast extract increased the final hir