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Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies

✍ Scribed by Anna Surribas; Rainer Stahn; José Luis Montesinos; Sven-Olof Enfors; Francisco Valero; Mehmedalija Jahic


Book ID
118012878
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
861 KB
Volume
130
Category
Article
ISSN
0168-1656

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The yeast Pichia pastoris has been previously used for extracellular expression of a Rhizopus oryzae lipase (Rol). However, limitations in Rol folding and secretion through the cell wall became apparent when producing it in fed-batch cultivations. In this study, we have investigated the effect of co