Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris
✍ Scribed by Stefan Minning; Alicia Serrano; Pau Ferrer; Carles Solá; Rolf D. Schmid; Francisco Valero
- Book ID
- 119523946
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 195 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0168-1656
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The yeast Pichia pastoris has been previously used for extracellular expression of a Rhizopus oryzae lipase (Rol). However, limitations in Rol folding and secretion through the cell wall became apparent when producing it in fed-batch cultivations. In this study, we have investigated the effect of co
for loadings higher than 10% in AlPO4-Sepiolite. FFA content increased with lipase load. Thermal oxidation was negligible. For all the biocatalysts tested, the catalytic activity increased with the aw. A 100% SFC35 • C reduction was observed after six hours of reaction, at aw of 1. However, the esti