Biochemical characterization and studies of adsorption and immobilization of recombinant Rhizopus oryzae lipase expressed in Pichia pastoris

✍ Scribed by M. Guillén; M.D. Benaiges; F. Valero

• Publisher: Elsevier
• Year: 2009
• Tongue: English
• Weight: 79 KB
• Volume: 25
• Category: Article
• ISSN: 1871-6784
• DOI: 10.1016/j.nbt.2009.06.393

✦ Synopsis

for loadings higher than 10% in AlPO4-Sepiolite. FFA content increased with lipase load. Thermal oxidation was negligible. For all the biocatalysts tested, the catalytic activity increased with the aw. A 100% SFC35 • C reduction was observed after six hours of reaction, at aw of 1. However, the estimated half-life time of the lipase in AlPO4-Sepiolite was only about 53 hours, when used in consecutive batches.