✦ LIBER ✦

Immobilization and stability of a Rhizopus oryzae lipase expressed in Pichia pastoris: Comparison between native and recombinant variants

✍ Scribed by Marina Guillén; Maria Dolors Benaiges; Francisco Valero

Publisher: American Institute of Chemical Engineers
Year: 2011
Tongue: English
Weight: 764 KB
Volume: 27
Category: Article
ISSN: 8756-7938
DOI: 10.1002/btpr.654

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Biochemical characterization and studies

Biochemical characterization and studies of adsorption and immobilization of recombinant Rhizopus oryzae lipase expressed in Pichia pastoris

✍ M. Guillén; M.D. Benaiges; F. Valero 📂 Article 📅 2009 🏛 Elsevier 🌐 English ⚖ 79 KB

for loadings higher than 10% in AlPO4-Sepiolite. FFA content increased with lipase load. Thermal oxidation was negligible. For all the biocatalysts tested, the catalytic activity increased with the aw. A 100% SFC35 • C reduction was observed after six hours of reaction, at aw of 1. However, the esti

Recombinant Candida rugosa lipase 2 from

Recombinant Candida rugosa lipase 2 from Pichia pastoris: Immobilization and use as biocatalyst in a stereoselective reaction

✍ M. Dolors Benaiges; Manuel Alarcón; Pablo Fuciños; Pau Ferrer; Marisa Rua; Franc 📂 Article 📅 2010 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 293 KB

High-level expression and stabilization

High-level expression and stabilization of recombinant human chitinase produced in a continuous constitutive Pichia pastoris expression system

✍ J. C. Goodrick; M. Xu; R. Finnegan; B. M. Schilling; S. Schiavi; H. Hoppe; N. C. 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 464 KB 👁 1 views

## Abstract A continuous fermentation process has been developed in __Pichia pastoris (P. pastoris__) with the glyceraldehyde‐3‐phosphate dehydrogenase (__GAP__) promoter in order to produce large quantities of recombinant human chitinase (rh‐chitinase) for preclinical studies as a potential high‐d