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The effect of glycerol mixed substrate on the heterologous production of a Rhizopus oryzae lipase in Pichia pastoris system

✍ Scribed by Carolina Arnau; Carles Casas; Francisco Valero


Book ID
113489811
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
815 KB
Volume
57
Category
Article
ISSN
1369-703X

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The yeast Pichia pastoris has been previously used for extracellular expression of a Rhizopus oryzae lipase (Rol). However, limitations in Rol folding and secretion through the cell wall became apparent when producing it in fed-batch cultivations. In this study, we have investigated the effect of co