Two hybridomas (PQXB1/2 and PQXB2/2) derived from the same parental cell lines showed a three times difference in specific antibody productivity. Measurement of the specific activities of three glycosyltransferase enzymes (galactosyl-, sialyl-and fucosyl-) showed significantly higher values (up to x
Production of a monoclonal antibody with specificity for the pellicle ofPneumocystis cariniiby hybridoma
โ Scribed by Y. Matsumoto; T. Amagai; M. Yamada; J. Imanishi; Y. Yoshida
- Publisher
- Springer-Verlag
- Year
- 1987
- Tongue
- English
- Weight
- 755 KB
- Volume
- 73
- Category
- Article
- ISSN
- 1432-1955
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Mouse hybridoma cells were succesfully cultivated for more than 640 hours in the interparticle spaces of a tubular reactor packed with spherical dass beads. The maximum monoclonal antibody (MAb) concentration attained was 110 mg/l and a viable cell density in the order of 1 X 1 O7 cells/ml was achie
A major variable to consider in the production of biologicals from mammalian cell cultures is the mode of operation, be it a batch, continuous, perfusion, fed-batch or other production method. The final choice must consider a number of fundamental and economic issues. Here we present some antibody p