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Specific activities of glycosyltransferase enzymes vary with monoclonal antibody productivity in murine hybridomas

โœ Scribed by Clair R. Cole; Christopher A. Smith; Michael Butler

Publisher
Springer Netherlands
Year
1993
Tongue
English
Weight
298 KB
Volume
15
Category
Article
ISSN
0141-5492

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โœฆ Synopsis

Two hybridomas (PQXB1/2 and PQXB2/2) derived from the same parental cell lines showed a three times difference in specific antibody productivity. Measurement of the specific activities of three glycosyltransferase enzymes (galactosyl-, sialyl-and fucosyl-) showed significantly higher values (up to x12) in the high secretor (PQXB1/2).

These results infer that high glycosylatoty activities are associated with effective monoclonal antibody secretion.

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