We previously compared changes in individual protein abundance between the proteomes of GS-NS0 cell lines with varying rates of cell-specific recombinant monoclonal antibody production (qMab). Here we extend analyses of our proteomic dataset to statistically determine if particular cell lines have d
โฆ LIBER โฆ
Comparative proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate
โ Scribed by C.M. Smales; D.M. Dinnis; S.H. Stansfield; D. Alete; E.A. Sage; J.R. Birch; A.J. Racher; C.T. Marshall; D.C. James
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 808 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0006-3592
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An amplified NS0 cell line transfected with a vector expressing a humanized monoclonal antibody (MAb) against CD-18 and glutamine synthetase (GS) was cultivated in a 1.5 L fed-batch culture using a serum-free, glutamine-free medium. Concentrated solutions of key nutrient components were fed periodic