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A novel immobilized hybridoma reactor for the production of monoclonal antibodies

✍ Scribed by O. T. Ramirez; R. Mutharasan; W. E. Magee

Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
388 KB
Volume
1
Category
Article
ISSN
0951-208X

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✦ Synopsis

Mouse hybridoma cells were succesfully cultivated for more than 640 hours in the interparticle spaces of a tubular reactor packed with spherical dass beads. The maximum monoclonal antibody (MAb) concentration attained was 110 mg/l and a viable cell density in the order of 1 X 1 O7 cells/ml was achieved. A productivity per reactor void volume of 5.2 mg MAb/hr/l was obtained, which is comparable to the best systems currently in use.

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## Abstract Cell proliferation and long‐term production of monoclonal antibody IgG~2b~ by M2139 hybridoma cells immobilized in macroporous gel particles (MGPs) in packed‐bed reactor were studied for a period of 60 days. The MGPs were made of supermacroporous gels produced in frozen conditions from