Enhancement of monoclonal antibody production by immobilized hybridoma cell culture with hyperosmolar medium

✍ Scribed by Sung Young Park; Gyun Min Lee

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 695 KB
• Volume: 48
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260480618

✦ Synopsis

To determine the effect of hyperosmotic stress on the monoclonal antibody (MAb) production by calciumalginate-immobilized S3H5iy2bA2 hybridoma cells, the osmolalities of medium in the M A b production stage were varied through the addition of NaCI. The specific MAb productivity (9Mab) of immobilized cells exposed to abrupt hyperosmotic stress (398 m0smikg) was increased by 55% when compared with that of immobilized cells in the control culture (286 m0smikg). Furthermore, this enhancement of qMAb was not transient. Abrupt increase in osmolality, however, inhibited cell growth, resulting in no increase in volumetric MAb productivity (rMAb). On the other hand, gradual increase in osmolality allowed further cell growth while maintaining the enhanced qMAb of immobilized cells. The qMAb of imrnobilized cells at 395 mOsmikg was 0.661 * 0.019 ~gI10' cellslh, which is almost identical to that of immobilized cells exposed to abrupt osmotic stress. Accordingly, the rMAb was increased by ca. 40% when compared with that in the control immobilized cell culture. This enhancement in rMAb of immobilized S3H5/y2bA2 hybridoma cells by applying gradual osmotic stress suggests the potential of using hyperosmolar medium in other perfusion culture systems for improved MAb production.