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Procedure for enzymatic synthesis and isolation of radioactive long chain acyl-CoA esters

✍ Scribed by Robert J. Banis; Cynthia S. Roberts; Gilbert B. Stokes; Samuel B. Tove

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
511 KB
Volume
73
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described that yields pure long chain fatty acyl-CoA esters. It utilizes a partially purified long chain acyl-CoA synthetase that can be obtained from various sources. Product isolation involves butanol extraction and thin-layer chromatography. It is applicable to a wide range of fatty acids and is particularly suitable in preparing radioactive acyl-CoA esters.

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