Procedure for the enzymatic synthesis and isolation of cinnamoyl-CoA thiolesters using a bacterial system
✍ Scribed by Meinhart H. Zenk; Bernhard Ulbrich; Jochen Busse; Joachim Stöckigt
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 437 KB
- Volume
- 101
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method is described which allows the preparation of pure cinnamoyl-CoA thiolesters in high yields. This procedure utilizes a partially purified cinnamoyl-CoA ligase obtained from a strain of fseudomonus putidu and some properties of this new enzyme are described. Product isolation involves polyamide column chromatography which allows the purification of SO-mg batches of thiolesters. The method is applicable to a range of cinnamic acids, and is particularly suitable in preparing the biologically important CoA esters of p-coumarate, ferulate. and caffeate. /C-S-COP, 0' EXPERIMENTAL PROCEDURES Chemicals.
Cinnamic acids were either purchased from Fluka (Buchs) or synthesized via the Knoevennagel reaction (4). Polyamide (MN polyamide SC 6, particle size 0.07 mm) was from Machery and Nagel