Preparation of β-carotene-14C

✍ Scribed by Albert E. Purcell; William M. Walter Jr.

• Publisher: John Wiley and Sons
• Year: 1968
• Tongue: French
• Weight: 139 KB
• Volume: 4
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2590040115

✦ Synopsis

In response to a need for relative large amounts of highly labeled 14C p carotene, a search was made for a biological system which would supply an adequate amount of carotene with sufficient label. Methods previously described did not appear to be adequate appear to devote a greater part of their metabolism to the synthesis of (3 carotene than do other systems. The results of growing mated cultures of Blakeslea trispora in the presence of 14C02, U14C glucose (Nuclear Chicago) * and l-P4C acetic acid (Nuclear Research Chemicals) are reported.

Blakeslea trispora 2895 and 2896, obtained from Northern Utilization Research Development Division, ARS, U. S. Department of Agriculture, were carried on potato dextrose agar slants. Five to 10 day old cultures were planted into 150 ml sterile 5 % pharmamedia * and 0.5% yeast extract in 500 ml erlenmyer flask stoppered with cotton. The flasks were incubated on a rotary shaker at 200 strokes per minute, 28-32O C for 48 hours. At that time 5 ml of each culture were transferred to lOOml of sterile media in 500ml flasks.