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Preparation of ribonuclease-free DNase I and α-amylase

✍ Scribed by Carleton T. Garrett; David S. Wilkinson; Henry C. Pitot

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
300 KB
Volume
52
Category
Article
ISSN
0003-2697

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✦ Synopsis

DNase I and cu-amylase are commercial products obtained from mammalian sources and may be separated from contaminating RNase by treatment. with bentonite. The completeness of RNase removal is demonstrated by using a highly sensitive technique employing E. coli ribosomal RKA as substrate.

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