Effects of isoflurane on the Dnase I activity in an isolated enzyme preparation and on the Dnase I-G actin complex

✍ Scribed by Reitz, Manfred ;Von Pfeil, Andrea ;Hundt, Ferdinand ;Lanz, Egon

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 562 KB
• Volume: 6
• Category: Article
• ISSN: 0887-2082
• DOI: 10.1002/jbt.2570060207

✦ Synopsis

Effects of isoflurane on the DNase I activity in an isolated enzyme preparation and in the DNase I-globular (G) actin complex were investigated. DNase I, DNase I-G actin complex, and G actin were exposed to various (0.2-4.0 ~0 1 % ) isoflurane concentrations for 180 min. Thereafter, DNase I activity was determined. DNase I activity was inhibited in relation to time and concentration of isoflurane exposure. At concentrations ranging from 0.2 to 1.0 vol% of isoflurane inactive DNase I was activated in the DNase I-G actin complex. The DNase I inhibitor G adin showed a reduced capability to inhibit DNase I following isoflurane exposure. Albumin can inhibit the DNase I inactivation possibly by competition in the reactions between DNase I/aL bumin and isoflurane. After exposure to isoflurane the absorption maximum of DNase I was identical with the absorption maximum of heat-denaturated DNase I. The results suggest a mechanism by which isoflurane may affect DNA in an indirect way at concentrations to which the patient is exposed during clinical anesthesia.