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Standard preparations of ribonuclease crystals of modifications I and II

✍ Scribed by Murray Vernon King

Publisher: Elsevier Science
Year: 1964
Weight: 316 KB
Volume: 79
Category: Article
ISSN: 0926-6577
DOI: 10.1016/0926-6577(64)90019-1

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✦ Synopsis

Detailed methods are described for preparation of bovine pancreatic ribonuclease (polyribonucleotide 2-ofigonucleotidotransferase (cyclizing), EC 2.7.7.16 ) crystals of Modifications I and II of quality suitable for X-ray diffraction studies. The reasons for choice of solvent and buffer systems are discussed, especial regard being paid to the use of the solvent 2-methyl-2,4-pentanediol and to the application of preserving solutions as media for handling the crystals after growth. Some necessary precautions are specified.

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