## Abstract A procedure for preparing polyacrylamide gel‐filled capillaries, for use in capillary gel electrophoresis, is decribed. The method involves the principle of isotachophoretic polymerization, in which the polymerization initiator is introduced by electromigration to promote gradual polyme
Preparation of highly condensed polyacrylamide gel-filled capillaries
✍ Scribed by Yi Chen; Joachim-Volker Höltje; Uli Schwarz
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 776 KB
- Volume
- 680
- Category
- Article
- ISSN
- 1873-3778
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## Abstract A new preparation method for polyacrylamide gels in fused silica capillaries is described. In this method use is made of riboflavin as a light sensitive initiator for the polymerization reaction. Also, results from measurements on the diffusion coefficients of four oligonucleotides in a
## Abstract Polyacrylamide gel filled capillaries for electrophoresis can be improved in performance and stability by a specially optimized and reproducible production procedure. After surface pretreatment as described in this contribution, gels polymerized in capillaries are stable for long series
+ a) elution possible bl elution possible only after derivatization C) no elution. ## 4 Conclusion Capillary electrophoresis can be used to separate (fluorinated) nucleosides and nucleotides and has proved to have advantages over capillary GC, SFC, and HPLC. Data obtained from ongoing References