The safety of a plasma-derived hepatitis-B vaccine inactivated by two heating steps (90 sec at 103 "C followed by 10 hr pasteurization at 65 "C) was validated in chimpanzees; 1 O3 chimpanzee-infectious doses (CID,,) of hepatitis-B virus (HBV), subjected to the purification steps during production of
Preparation of hepatitis B vaccine
โ Scribed by H. G. J. Brummelhuis
- Publisher
- Springer Netherlands
- Year
- 1983
- Tongue
- English
- Weight
- 63 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0003-6072
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## Abstract The immunogenicity of three experimental hepatitis B vaccines was evaluated in chimpanzees. Although no antibody to hepatitis B surface antigen (antiโHBs) was detected in two chimpanzees that received an aqueous polypeptide vaccine subcutaneously, a strong antiโHBs response was observed
A method for obtaining a subunit inactivated vaccine preparation from the 22-nm particles of HBsAg is proposed. For inactivation of the residual infectious hepatitis B virus (HBV) the preparations were successively treated with 1% sodium dodecyl sulfate (SDS) and nucleases. In addition, thermal dena