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Inactivation of 1015 chimpanzee-infectious doses of hepatitis B virus during preparation of a heat-inactivated hepatitis B vaccine

✍ Scribed by Dr. P. N. Lelie; H. W. Reesink; J. Niessen; B. Brotman; A. M. Prince

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 396 KB
Volume: 23
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890230312

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✦ Synopsis

The safety of a plasma-derived hepatitis-B vaccine inactivated by two heating steps (90 sec at 103 "C followed by 10 hr pasteurization at 65 "C) was validated in chimpanzees; 1 O3 chimpanzee-infectious doses (CID,,) of hepatitis-B virus (HBV), subjected to the purification steps during production of the vaccine, were noninfectious in two chimpanzees. Furthermore, lo6 CID,, of HBV heated at 103 "C for 90 sec and another lo6 CID,, of HBV heated at 65 "C for 10 hr, were also not infectious in two other chimpanzees. All animals developed hepatitis-B infection after subsequent challenge with the untreated starting material, before the respective purification and inactivation procedures. Thus, the total reduction of infectivity of HBV during production of this vaccine was established to be at least lo', CID,,.

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