𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A vaccine prepared from the 22 nm particles of surface hepatitis B antigen (HBsAg)

✍ Scribed by Dr. V. P. Karelin; E. E. Babaeva; E. F. Gubenko; D. K. Kaulen; V. M. Zhdanov

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
700 KB
Volume
5
Category
Article
ISSN
0146-6615

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✦ Synopsis

A method for obtaining a subunit inactivated vaccine preparation from the 22-nm particles of HBsAg is proposed. For inactivation of the residual infectious hepatitis B virus (HBV) the preparations were successively treated with 1% sodium dodecyl sulfate (SDS) and nucleases. In addition, thermal denaturation and ultraviolet irradiation of HBV DNA were used. As a control the biologic activity of a reference virus (SV40) was tested after the same treatment. The effectiveness of DNA inactivation was monitored by adding H-thymidine labeled reference virus to the vaccine preparations. The purified and inactivated HBsAg was adsorbed on All 0 3 . Antigenicity was calculated on the basis of the determination of antibody in guinea pigs immunized with various doses of the vaccine, and the release of 12s I-HBsAg from blood and kidneys in immunized and control mice was analyzed. Possible methods of inactivation and control of HBV vaccine is discussed.

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