A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d
Preparation of Escherichia coli elongation factor tu-guanosine 5′-triphosphate analogs
✍ Scribed by Katherine Delaria; Frances Jurnak
- Book ID
- 102986380
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 677 KB
- Volume
- 177
- Category
- Article
- ISSN
- 0003-2697
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Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in the presence of novel inhibitors. The only crystals which could be grown were epitaxially as well as merohedrally twinned, highly mosaic and diffracted to a resolution of 3.4 A ˚in space group P3 1 21, with unit-cell parameter