Visualization of elongation factor Tu on the Escherichia coli ribosome
β Scribed by Heel, Marin van; Stark, Holger; Rodnina, Marina V.; Rinke-Appel, Jutta; Brimacombe, Richard; Wintermeyer, Wolfgang
- Book ID
- 109799814
- Publisher
- Nature Publishing Group
- Year
- 1997
- Tongue
- English
- Weight
- 593 KB
- Volume
- 389
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/38770
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The str operon of Escherichia coli contains genes for ribosomal proteins S12 and S7 and for elongation factors EF-G and EF-Tu (Jaskunas et al. 1975). We have subcloned various segments of DNA from this operon onto multicopy plasmids. We found that cells carrying a recombinant plasmid which lacks the
A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d
Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in the presence of novel inhibitors. The only crystals which could be grown were epitaxially as well as merohedrally twinned, highly mosaic and diffracted to a resolution of 3.4 A Λin space group P3 1 21, with unit-cell parameter