A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d
The identification and analysis of nucleotides bound to the elongation factor Tu from Escherichia coli
β Scribed by Wolfgang Block; Alfred Pingoud
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 429 KB
- Volume
- 114
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The gluconeogenic phosphoenolpyruvate (PEP) carboxykinase is active in __Escherichia coli__ during its growth on glucose. The present study investigated the influence of growth rates and PEP carboxykinase knockout on the anaplerotic fluxes in __E. coli__. The intracellular fluxes were d
## Abstract In a previous report we described a multivariate approach to discriminate between the different response mechanisms operating in __Escherichia coli__ when a steady, continuous culture of these bacteria was perturbed by a glycerol pulse (Guebel et al., 2009, Biotechnol Bioeng 102: 910β92