Preparation of [β-32P]ribonucleoside-5′-triphosphates using permeable cells of Escherichia coli

Use of actin-bound adenosine 5′-diphosph

Use of actin-bound adenosine 5′-diphosphate as a method to determine the specific 32P-radioactivity of the γ-phosphoryl group of adenosine 5′-triphosphate in a highly compartmentalized cell, the platelet

✍ Adrie J.M. Verhoeven; Carol A. Cook; Holm Holmsen 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 609 KB

Determination of the specific 32P-radioactivity of cytoplasmic ATP in '\*P-Pi-labeled platelets is complicated by the presence of a large pool of metabolically inactive, granule-stored nucleotides. Moreover, our data show that the specific 32P-radioactivity of cytoplasmic ATP is severely underestima