Practical aspects of the 2D15N-{1H}-NOE experiment
β Scribed by Christian Renner; Michael Schleicher; Luis Moroder; Tad A. Holak
- Book ID
- 110336493
- Publisher
- Springer Netherlands
- Year
- 2002
- Tongue
- English
- Weight
- 261 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0925-2738
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π SIMILAR VOLUMES
A 3D NOESY-(HCACO)NH experiment is described that transfers NOEs from 1H alpha to the backbone 1Hn in the succeeding residue for detection. Using this strategy, NOEs involving 1H alpha protons that resonate exactly at the water frequency can be detected. NOEs from an overlapping 1H alpha proton that
A simple method is presented to accurately determine 15 N-{ 1 H} NOEs in biomolecules in the presence of H(N)-water proton chemical exchange. Three measurements are required: one with nonselective proton saturation and two with different water saturation conditions to determine the equilibrium value