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Improved Measurement of 15N–{1H} NOEs in the Presence of H(N)-Water Proton Chemical Exchange

✍ Scribed by Djaudat Idiyatullin; Vladimir A. Daragan; Kevin H. Mayo

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
146 KB
Volume
153
Category
Article
ISSN
1090-7807

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✦ Synopsis

A simple method is presented to accurately determine 15 N-{ 1 H} NOEs in biomolecules in the presence of H(N)-water proton chemical exchange. Three measurements are required: one with nonselective proton saturation and two with different water saturation conditions to determine the equilibrium value of the 15 N signal. This approach is exemplified with data on two peptides, one helixforming 17-mer and one compactly folded 56-mer. Results indicate that 15 N-{ 1 H} NOEs determined using the standard approach with short recycle times (3 to 4 s) can be significantly in error when H(N)water proton chemical exchange is relatively rapid, water proton relaxation is relatively slow, and 15 N-{ 1 H} NOEs are away from the value of -1. This new method avoids such inaccuracies resulting from the use of short recycle times.

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