Overcoming Solvent Saturation-Transfer A
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Y.C. Li; G.T. Montelione
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Article
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1994
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Elsevier Science
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English
โ 525 KB
Artifacts due to solvent saturation-transfer effects result in incorrect measurements of 1H-15N heteronuclear NOE (HNOE). These artifacts are commonly observed in aqueous protein solutions at neutral pH. We describe the application of pulsed field gradients (PFGs) together with long recycle delays i