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Overcoming the Solvent-Saturation Transfer Problem in 1H- 15N NOE Measurements. Application of Jump-Return Pulses

โœ Scribed by M.D. Sorensen; S.M. Kristensen; J.J. Led


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
374 KB
Volume
107
Category
Article
ISSN
1064-1866

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Overcoming Solvent Saturation-Transfer A
โœ Y.C. Li; G.T. Montelione ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 525 KB

Artifacts due to solvent saturation-transfer effects result in incorrect measurements of 1H-15N heteronuclear NOE (HNOE). These artifacts are commonly observed in aqueous protein solutions at neutral pH. We describe the application of pulsed field gradients (PFGs) together with long recycle delays i