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Modern proton-detected 1D 1H–15N NMR experiments. Application to the measurement of 1H,15N coupling constants at natural abundance

✍ Scribed by Teodor Parella; Jordi Belloc

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
151 KB
Volume
40
Category
Article
ISSN
0749-1581

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✦ Synopsis

Abstract

Non‐selective and selective versions of several proton‐detected 1D NMR experiments to be applied to ^15^N are proposed. Clean, artifact‐free 1D spectra are easily obtained by the effective coherence selection by pulsed‐field gradients and the attainable sensitivity is maximized using modern pulse schemes. Despite the low sensitivity inherent to ^15^N NMR spectroscopy, the successful application of these experiments is demonstrated for resonance assignments and accurate measurement of both one‐bond and long‐range proton–nitrogen coupling constants on a model tripeptide at natural abundance. Copyright © 2001 John Wiley & Sons, Ltd.

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